Tissue Culture Plant Acclimation
![Tissue Culture Plant Acclimation](/content/images/size/w2000/2022/09/304849463_1226911068158015_2710082736260895463_n.png)
![](https://www.itspersonal.day/content/images/2022/09/p-white-wizard.jpg)
![](https://www.itspersonal.day/content/images/2022/09/p-white-wizard-back.jpg)
Recently I took the gamble of trying out tissue culture baby plants off a Facebook plant group. They were fairly cheap as the white wizard philodendron x10 tissue culture pups' agar was molding. I had also bought a white princess philodendron x10 pups.
FYI- I did not follow ALL the steps I should have to make sure the transition goes properly and I do not lose a single pup but we will see how it goes.
![](https://www.itspersonal.day/content/images/2022/09/p-white-princess.jpg)
![](https://www.itspersonal.day/content/images/2022/09/p-white-princess-back.jpg)
To be honest I didn't mean to win the white princess sale but the person above me deleted their comment so I became the second in line to win...
Anyways, from the instructions these plant pups have only been in sterile conditions. Everything I had done from washing, potting, and drying had to all be fairly sterile.
![](https://www.itspersonal.day/content/images/2022/09/wpp-ball.jpg)
In order to pull apart each plant pup, I had to use tweezers to carefully remove agar and untangle roots. Some leaves and roots definitely were lost in the process. I also had to remove any dead/yellowing leaves.
![](https://www.itspersonal.day/content/images/2022/09/tc.jpg)
During the potting up process, I microwaved the sphagnum moss with perlite in distilled water to kill off any bacteria. I placed it in an upside down clear container to create a humidity dome. Everything was also sanitized to make sure I mimicked that sterile and 100% sealed humidity which the plant pups had originally came in so that they can acclimate properly.
Unfortunately I did not have any solutions/chemicals on hand to help sterilize the pups any further . I mainly used clorox wipes and hand sanitizer spray.....So as I am posting this now.. After a week the white knights are battling a mold issue on the roots.. Oops...I will post again in a week.
![](https://www.itspersonal.day/content/images/2022/09/humidity-dome2.jpg)
![](https://www.itspersonal.day/content/images/2022/09/humidity-dome-1.jpg)
And now we wait a few weeks and see how it goes!